Why Is Pcr Used In The Process Of Dna Sequencing Quizlet
Olivia Luz
![Why Is Pcr Used In The Process Of Dna Sequencing](https://users.ugent.be/~avierstr/principles/seqsteps.gif)
Because significant amounts of a sample of dna are necessary for molecular and genetic analyses studies of isolated pieces of dna are nearly impossible without pcr amplification.
Why is pcr used in the process of dna sequencing. How can dna sequencing technique shown in the virtual lab be used to identify other classes of pathogens such as viruses. The pcr is a process employed to amplify the dna and used in the dna sequencing as well to get dna copies to reduce contamination identify dna mutations and recombinant clones in the conventional pcr method in order to get copies of dna our gene of interest or dna is amplified enzymatically using the forward and reverse primer sets. Generally the four types of dntps are incorporated into the newly synthesizing dna strand during pcr.
2 how can dna sequencing be used to identify other classes of pathogens such as viruses. Creates ideal temperature where the certain enzyme and primers can work to copy. The pcr is able to replicate the given dna into millions of copies and use only the smaller pieces of dnarequired to make a sequence. This is the ability to isolate strands from viruses and compare them to databases if desired.
Sometimes called molecular photocopying the polymerase chain reaction pcr is a fast and inexpensive technique used to amplify copy small segments of dna.
Source : pinterest.com